Il-18 Human Matched Antibody Pair, Matched Antibody Pair, Each

Details:

The human il-18 matched antibody pair kit comes with pre-matched antibody pairs,standards,sample diluent and streptavidin-hrp to develop your own enzyme linked immunosorbent assays (elisa) to detect and quantify protein levels of human il-18. Buffer reagents and microplates needed to complete the elisa reaction are sold separately. Principle of the method elisas are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate,which is an overnight process. Samples,standards,or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody,a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.The protein encoded by this gene is a proinflammatory cytokine. This cytokine can induce the ifn-gamma production of t cells. The combination of this cytokine and il12 has been shown to inhibit il4 dependent ige and igg1 production,and enhance igg2a production of b cells. Il-18 binding protein can specifically interact with this cytokine,and thus negatively regulate its biological activity.