Il-21 Rabbit Anti-Human, Rabbit Recombinant Polyclonal Antibody, Each

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Intact igg appears on a non-reducing gel as ∽150 kda band and upon reduction generating a ∽25 kda light chain band and a ∽50 kda heavy chain. Recombit rabbit polyclonal antibodies are unique offerings from thermo fisher scientific. They are comprised of a selection of multiple different recombit monoclonal antibodies, providing the best of both worlds – the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombit antibody. While functionally the same as a polyclonal antibody – recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets – a recombit rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.Il-21 is a 17 kda immunomodulatory cytokine produced mainly by natural killer cells (nkt), t helper (th) 17 and t follicular helper (tfh) cells. In tfh cells, il-21 expression leads to autocrine signaling through the il-21 receptor (il-21r) and stat3, which leads to additional transcriptional activation by bcl6. As with ifn gamma for th1 and il-4 for th2 cells, il-21 is critical for tfh cell development and effector function. Il-21 plays a role in t cell-dependent b cell differentiation into plasma cells and memory cells, stimulation of igg production and induction of apoptotic signaling in naive b cells. In th17 cells, il-21 expression and autocrine feedback through stat3, irf4 and ror gamma t lead to upregulation of the il-23r, thereby preparing th17 cells for maturation and maintece by the inflammatory cytokine il-23. While upregulating irf4 and ror gamma t, il-21 also mediates the downregulation of foxp3. Il-21 deficient mice are protected from developing colitis upon chemical treatment by their inability to upregulate th17-associated molecules. In comparison, elevated levels of il-21 are present in chemically-induced colitis mouse models.