Il-4 Porcine Matched Antibody Pair, Matched Antibody Pair, Each

Details:

The porcine interleukin 4 antibody pair kit comes with pre-matched antibody pairs, standards, and streptavidin-hrp to develop your own enzyme linked immunosorbent assays (elisa) to detect and quantify protein levels of pig il-4. Buffer reagents needed to complete the elisa reaction are sold separate as a buffer kit for antibody pairs (catalog no. Cnb0011). The assay buffer included in this buffer kit can be used as a blocking reagent for elisa plates as well as a diluent for elisa standards and samples, detection antibody, and hrp conjugate. Principle of the method elisas are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.The protein encoded by this gene is a pleiotropic cytokine produced by activated t cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to il13, which may contribute to many overlapping functions of this cytokine and il13. Stat6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, il3, il5, il13, and csf2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to il13. This gene, il13 and il5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.