Il-8 Human Matched Antibody Pair, Matched Antibody Pair, Each

Details:

The human il-8/nap-1 matched antibody pair kit comes with pre-matched antibody pairs,standards,and streptavidin-hrp to develop your own enzyme linked immunosorbent assays (elisa) to detect and quantify protein levels of human il-8. Buffer reagents and microplates needed to complete the elisa reaction are sold separately. Principle of the method elisas are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate,which is an overnight process. Samples,standards,or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody,a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.Il-8 (interleukin-8) is a 72 amino acid pro-inflammatory cxc chemokine designated cxcl8,secreted by endothelial cells and monocytes,which acts as a major chemoattractant for neutrophils and also t cells and basophils. Il-8,first isolated from osteosarcoma cells,contains the elr-motif (n-terminal glu-leu-arg amino acid sequence) and signals through the cxcr1 and cxcr2 receptors. Cancer studies have demonstrated a role for il-8 in the angiogenesis and growth of tumours. Previous nomenclature for il-8 includes neutrophil activating protein 1 (nap-1),granulocyte chemotactic protein 1 (gcp-1),monocyte-derived neutrophil-activating peptide (monap) and protein 3-10c.