Irs-1 Mouse, Unlabeled, Clone: 6, Bd, Mouse Monoclonal Antibody, Each

Details:

The irs (insulin receptor substrate) proteins irs-1, irs-2, irs-3, and irs-4 are major substrates of the insulin receptor tyrosine kinase and the insulin-like growth factor-1 receptor. Irs proteins contain an n-terminal pleckstrin homology (ph) domain, an atp-binding domain, and multiple tyrosine phosphorylation sites in the c-terminus. Following insulin receptor ligation, irs-1 binds to the juxtamembrane region of the receptor and is tyrosine phosphorylated. This facilitates its interaction with sh2 domain-containing signaling proteins, such as pi3 kinase, fyn, grb2, and ptp1d. Phosphorylation dramatically reduces the affinity of irs-1 for the insulin receptor, indicating that dissociation from the receptor and subsequent subcellular translocation are important to irs-1 function in the pleiotropic effects induced by insulin. In support of this, irs-1-null mice are viable, but exhibit growth retardation and abnormal glucose metabolism. In cases of reduced irs-1 expression, certain irs-1 functions can be assumed by the related irs-2 protein, while other activities linked to irs-1 are inhibited. Thus, irs-1 is an essential component of insulin induced signal transduction.Immunofluorescence, western blotting