Jti R4 Cmv-To Mcs Pa Vector, Pjti R4 Cmv-To Mcs Pa Vector, Each

Details:

The pjti™ r4 cmv-to mcs pa vector is designed for the expression of your gene of interest under the control of the tet-inducible cmv promoter after restriction enzyme cloning and retargeting into the genomic r4 site of a jump-in™ parental cell line. This vector can be used for inducible or constitutive expression of your gene of interest, depending on which jump-in™ parental cell line you use. When this vector is retargeted into a jump-in™ t-rex™ parental cell line, gene expression is controlled by the tet-operon and can be induced by adding doxycycline to the growth media. When used with a jump-in™ parental cell line such as the jump-in™ griptite™ hek293 cell line, constitutive expression is achieved after retargeting. The r4 sites in the jump-in™ parental cell lines result in a high retargeting efficiency, requiring less effort and less time than traditional cell engineering methods. Retargeting of jump-in™ parental cell lines results in creation of an isogenic pool that is sufficient for cell-based experiments without the need for clonal selection. Alternatively, the high retargeting efficiency allows for easy selection of a positive stable clone for expressing your gene of interest.