Kpni, Hc (50 U/ΜL), Fermentas Kpni Is Available As A Fastdigest Enzyme Which Is Used For Rapid Dna Digestion, Each

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Thermo scientific™ kpni is available as a fastdigest enzyme which is used for rapid dna digestion. More than 95% of the digested dna fragments can be ligated and recut at a 5′-termini concentration of 0.02μM after a 50-fold overdigestion (3u/μg dna x 17 hours) with kpni.5'..G g t a c▵c...3'3'..C▵c a t g g...5'Conditions for 100% activity:1x buffer kpni: 10mm tris-hcl (ph 7.5 At 37°c), 10mm mgcl2, 0.02% Triton x-100 and 0.1Mg/ml bsaincubate at 37°cstorage buffer:kpni is supplied in: 10mm tris-hcl (ph 7.5 At 25°c), 50mm kcl, 1mm dtt, 0.1Mm edta, 0.2Mg/ml bsa and 50% (v/v) glycerolmethylation effects:dam: never overlaps —no effectdcm: may overlap —no effectcpg: may overlap —no effectecoki: never overlaps —no effectecobi: never overlaps —no effectdigestion of agarose-embedded dna:minimum 5u of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda dna in 16 hoursnote:high glycerol (>5%) concentrations, ph>8.0 Or a large excess of enzyme may result in star activity.