L-Lactic Dehydrogenase From Rabbit Muscle Type Ii, Ammonium Sulfate Suspension, 800-1,200 Units/mg Protein, 50 Ku, Each

Details:

Specifications synonyms: lactate; (s)-lactate: nad oxidoreductase; l-ldh; lad; ldcas number: 9001-60-9mdl no.: Mfcd00131466storage: 2-8cenzyme commission (ec) number: 1.1.1.27  ( Brenda | iubmb )ec number: 232-617-8unspsc code: 12352204general description: lactic dehydrogenase (ldh) has a total molecular weight of 140 kda and is composed of 4 subunits which are designated m subunit (muscle) and h subunit (heart). These subunits may be mixed in any of 5 combinations (m4, m3h1, m2h2, mh3, and h4). Skeletal muscle contains ldh that is predominately m4 with some small amounts of m3h and traces of h2h2. The h and m subunits are quite similar in molecular weight, but differ substantially in amino acid composition. Rabbit muscle ldh dissociates into dimeric species (mw = ~70 kda) in acetate-chloride at ph 5.0, The dissociation is reversible. Biochemistry, 13, 3527-3531 (1974). Oxidizes glyoxylate and lactate.Isoelectric point: 8.4-8.6Optimal ph : 7.5 .Biochem/physiol actions: also catalyzes the oxidation of other l-2-hydroxymonocarboxylic acids.Physical form: crystalline suspension in 3.2 M (nh4)2so4 solution, ph 6.0Unit definition: one unit will reduce 1.0 Mumole of pyruvate to l-lactate per min at ph 7.5 At 37 c.Analysis note: protein determined by biuret.Ridadr: nonh for all modes of transportwgk germany: 3