Mccoy'S 5a (Modified) Medium, Supports Propagation Of Many Types Of Primary Cells, Established Cell Lines, And Explants From Biopsy Tissues, Case Of 10

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Mccoy's 5a (modified) medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues. This mccoy's 5a is modified as follows: with high glucose, l-glutamine, bacto-peptone, phenol red; without sodium pyruvate, hepes.Modification of basal medium 5acontains reducing agent glutathione, bacto-peptone, and a high level of glucoseincludes dr. Hsu's addition of hank's salts to enable use outside co2 incubatorrequires serum supplementation, commonly with 10% fetal bovine serum (fbs)uses sodium bicarbonate buffer system (2.2G/l), and therefore requires 5-10% co2 environment to maintain physiological phsustainable packagingcell culture, mammalian cell culture