Mem Α, Nucleosides, Minimum Essential Medium Is Widely Used For Mammalian Cell Culture As Well As Selection For Transfected Dhfr-Negative Cells, Case Of 6

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Mem α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. This mem is modified as follows: ribonucleosides, deoxyribonucleosides, phenol red, l-glutamine.Modification of minimum essential medium (mem) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin b12, biotin, and ascorbic acidavailable without nucleosides for use as a selection medium for dg44 and other dhfr-negative cellsmade with earle's saltscontains no proteins, lipids, or growth factorsrequires supplementation, commonly with 10% fetal bovine serum (fbs)uses sodium bicarbonate buffer system (2.2G/l), and therefore requires 5-10% co2 environment to maintain physiological phsustainable packagingcell culture, mammalian cell culture