Mem, Minimum Essential Medium Is One Of The Most Commonly Used Of All Cell Culture Media, Case Of 10

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Mem (minimum essential medium) is one of the most commonly used of all cell culture mediamem can be used with a variety of suspension and adherent mammalian cells including hela, bhk-21, 293, hep-2, ht-1080, mcf-7, fibroblasts, and primary rat astrocytesstore at 2°c to 8°c, protected from lightmodified as follows:with: l-glutamine, phenol redwithout: hepesusing memthis product is made with earle’s saltsmem contains no proteins, lipids, or growth factors therefore mem requires supplementation, commonly with 10% fetal bovine serum (fbs)mem uses a sodium bicarbonate buffer system (2.2 G/l), and therefore requires a 5–10% co2 environment to maintain physiological phorder infoshipping condition: room temperature