Deoxyribonuclease I From Bovine Pancreas, Mp Biomedicals, Deoxyribonuclease I From Bovine Pancreas 50,000 Dornase U/mg Solid, White Powder, Each

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Deoxyribonuclease from beef pancreas, dnase i, was first crystallized by kunitz. It is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding 5'-phosphate terminated polynucleotides with a free hydroxyl group on position 3'. The average chain of limit digest is a tetranucleotide. Dnase i acts upon single chain dna, and upon double-stranded dna and chromatin. In the latter case, although histones restrict susceptibility to nuclease action, over a period of time nearly all chromatin dna is acted upon. According to mirsky and silverman, this could result from the looseness of histone attachment to dna. They found that lysine-rich histones more effectively block dnase access to dna than arginine-rich histone