Nos Mouse, Unlabeled, Clone: 16, Bd, Mouse Monoclonal Antibody, Each

Details:

Nitric oxide synthase (nos), a cell-type specific enzyme, catalyzes the synthesis of nitric oxide (no). No is a short-lived radical that transmits cellular signals involved in vasorelaxation, neurotransmission, and cytotoxicity. In neurons and endothelial cells, constitutive nos (cnos) is activated by agonists that increase intracellular ca2+ levels and enhance calmodulin binding. Neuronal nos (nnos or bnos) and endothelial nos (ecnos) have recognition sites for nadph, fad, fmn, and calmodulin and are regulated in a similar manner. However, both have been shown to be distinct gene products of about 155kda and 140kda, respectively, and the human forms show 52% amino acid identity. Neuronal nos and induced macrophage nos (inos) share 51% amino acid homology with the greatest degree of divergence in the calmodulin binding domain. Neuronal nos, a cytosolic protein present mainly in neural tissues, has been purified and characterized from rat cerebellum. The no synthesized by this enzyme acts as a neurotransmitter. Ecnos has been cloned from human vascular endothelium as well as from bovine aortic endothelial cells (baec) and has a unique n-myristylation consensus sequence that may explain its membrane localization.Immunofluorescence, immunohistochemistry, immunoprecipitation, western blotting