Noti, Hc (50 U/ΜL), Fermentas Noti Is Available As A Fastdigest Enzyme Which Is Used For Rapid Dna Digestion, Each

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Thermo scientific™ noti is available as a fastdigest enzyme which is used for rapid dna digestion, more than 95% of the digested dna fragments can be ligated and recut at a 5′-termini concentration of 0.5μM after a 50-fold overdigestion (3u/μg dna x 17 hours) with noti.5'..G c▵g g c c g c...3'3'..C g c c g g▵c g...5'Conditions for 100% activity:1x buffer o: 50mm tris-hcl (ph 7.5 At 37°c), 10mm mgcl2, 100mm nacl and 0.1Mg/ml bsaincubate at 37°cstorage buffer:noti is supplied in: 20mm tris-hcl (ph 7.8 At 25°c), 100mm nacl, 0.1Mm edta, 1mm dtt, 0.02% Triton x-100, 0.2Mg/ml bsa and 50% (v/v) glycerolligation and recleavage:after 50-fold overdigestion with noti, more than 95% of dna fragments can be ligated and recutmethylation effects:dam: never overlaps —no effectdcm: never overlaps —no effectcpg: completely overlaps —blockedecoki: never overlaps —no effectecobi: never overlaps —no effectdigestion of agarose-embedded dna:minimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded adenovirus-2 dna in 16 hourscompatible ends:bsp120i, cfri, eco52i note:assayed using ptz19rjl2 plasmid dna digested with bseli. Supercoiled plasmids may require up to 5-fold more noti for complete digestion than linear dnas.