Optizyme Rsai, Fisher Bioreagents, Each

Details:

5'...G t^a c...3'3'...C a^t g...5'Supplied with: 10x optizyme buffer 4conditions for 100% activity:1x optizyme buffer 4: 33mm tris-acetate (ph 7.9 At 37°c), 10mm mg-acetate, 66mm k-acetate and 0.1Mg/ml bsaincubate at 37°cenzyme activity in optizyme buffers:buffer 1: 50 - 100%buffer 2: 20 - 50%buffer 3: 0 - 20%buffer 4: 100%buffer 5: 0 - 20%storage buffer:10mm tris-hcl (ph 7.4 At 25°c), 100mm kcl, 1mm dtt, 1mm edta, 0.2Mg/ml bsa and 50% (v/v) glycerolligation and re-cleavage:more than 95% of the dna fragments can be ligated and re-cut after a 50-fold over-digestion with rsaimethylation effects:dam: never overlaps - no effectdcm: never overlaps - no effectcpg: may overlap - cleavage impaired5'...Gtam5c g...3'3'...Cat gm5c...5' (Cleavage is impaired)5'...M5c gtam5c g...3'3'... Gm5cat gm5c...5' (Cleavage is blocked)ecoki: never overlaps - no effectecobi: never overlaps - no effect