Optizyme Ddei (Hpyf31), Fisher Bioreagents, Each

Details:

5'...C^t n a g...3'3'...G a n t^c...5'Supplied with: 10x optizyme buffer 4conditions for 100% activity:1x optizyme buffer 4: 33mm tris-acetate (ph 7.9 At 37°c), 10mm mg-acetate, 66mm k-acetate and 0.1Mg/ml bsaincubate at 37°cenzyme activity in optizyme buffers:buffer 1: 20 - 50% buffer 2: 20 - 50% buffer 3: 20 - 50% buffer 4: 100% buffer 5: 20 - 50% storage buffer:10mm tris-hcl (ph 7.4 At 25°c), 100mm kcl, 1mm dtt, 1mm edta, 0.2Mg/ml bsa and 50% (v/v) glycerolligation and re-cleavage:more than 95% of the dna fragments can be ligated and re-cut after 50-fold over-digesiton with ddei.Compatible ends: bbvci, bpu10i, bpu1102i, eco81imethylation effects:dam: never overlaps - no effectdcm: never overlaps - no effectcpg: never overlaps - no effectecoki: never overlaps - no effectecobi: may overlap - effect not determined