27[Kip1] Mouse, Unlabeled, Clone: 57, Bd, Mouse Monoclonal Antibody, Each

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Kip1 is a cyclin-dependent kinase (cdk) inhibitor that was identified as a result of its role in tgfβ-induced g1 phase arrest and cell-cell contact. In vitro , p27 [kip1] binds tightly to cyclin d-cdk4, cyclin e-cdk2, and cyclin a-cdk2 complexes and inhibits their activity. In normal cells, kip1 is sequestered and its activity gradually decreases as cells reach s phase. The addition of tgf-β in early g1 blocks this decline in activity, by preventing the sequestration of kip1. Tgf-β also reduces the levels of cdk4. Kip1 preferentially binds cyclin d-cdk4, but the lower levels of cdk4 in tgf-β treated cells allow kip1 to be available for binding to cyclin e-cdk2 and cyclin a-cdk2. Kip1 is structurally related to cip1/waf1, having a similar 60 amino acid sequence in the n-terminal region. A 52 amino acid kip1 peptide (residues 28-79) from this region is sufficient to inhibit cdk activity in vitro.Immunofluorescence, immunohistochemistry, immunoprecipitation, western blotting