Pgfm Enzyme Competitive Elisa Kit, Competitive Elisa, Each

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The pgfm enzyme elisa quantitates pgfm in serum,plasma,urine,dried fecal extracts,or cell culture medium.Principle of the methodthe pgfm competitive elisa research-use-only kit designed to quantitatively measure pgfm present in fecal extracts,urine,serum,and plasma samples. A pgfm standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit igg. A pgfm-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a rabbit polyclonal antibody highly specific to pgfm to each well. After a 1-hour incubation,the plate is washed and substrate is added. The substrate reacts with the bound pgfm-peroxidase conjugate. After a short incubation,the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.Rigorous validationeach manufactured lot of this elisa kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.In many species, uterine and placental prostaglandin f2alpha (pgfm) is involved in the regulation of reproductive and pregnancy-related processes such as embryonic development, initiation of parturition, and resumption of ovarian activity. Prostaglandin f2alpha is metabolized to pgfm during the first passage through the lungs. Pgfm has a longer half-life in peripheral circulation than pgf2alpha and has been applied as a useful analytical marker of pgf2alpha. Pgfm is identical across all species and so this kit should measure pgfm from sources other than those tested.