1-Step Pnpp Substrate Solution, An Alkaline Phosphatase Substrate For Elisa Applications, Each

Details:

Pnpp (p-nitrophenyl phosphate, disodium salt) is a widely used substrate for detecting alkaline phosphatase in elisa applications. When alkaline phosphatase and pnpp are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405nm. Pierce pnpp is available in four formats: dry crystalline powder, single-use tablets, a stable substrate solution and a complete kit containing substrate solution and diethanolamine buffer.Highlights:absorbance maximum of 505nm for measurement by elisa plate readersdevelops a yellow hue in solution upon reaction with alkaline phosphatase (ap)safer to use – solution contains a non-mercury, non-azide preservative