Il-6 Bovine Uncoated Elisa Kit, Uncoated Elisa Kit, Each

Details:

The bovine il-6 uncoated elisa kit contains pre-matched antibody pairs and reagents for performing quantitative enzyme linked immunosorbent assays (elisa) to detect and quantify protein levels of bovine il-6. Principle of the method elisas are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.Interleukin 6 (il-6) is a multifunctional 26 kd protein originally discovered in the medium of rna-stimulated fibroblastoid cells. Il-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be important as il-1 and tnf-a in regulating the acute phase response. Il-6 is reported to be produced by fibroblasts, activated t cells, activated monocytes or macrophages and endothelial cells. Il-6 acts upon a variety of cells including myeloid progenitor cells, t cells, b cells, and hepatocytes. In addition, il-6 appears to interact with il-2 in the proliferation of t lymphocytes. Il-6 potentiates the proliferative effect of il-3 on multipotential hematopoietic progenitors. Il-6 plays a critical role in b-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. Il-6 is a very useful culture supplement for the generation of a high number of antibody-producing hybridomas. Primarily produced at sites of acute and chronic inflammation, il-6 is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of il-6 is implicated in a wide variety of inflammation-associated disease states including diabetes mellitus and systemic juvenile rheumatoid arthritis.