Pierce Ecl 2 Western Blotting Substrate, Recommended Hrp Substrate For Fluorescence Imagers, Each

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Pierce ecl 2 substrate is a chemiluminescent and chemifluorescent substrate to detect peroxidase activity from hrp-conjugated antibodies and probes by western blotting using x-ray film, ccd camera documentation systems or laser-based imagers. Pierce ecl 2 substrate generates acridinium esters when it reacts with hrp. As these ester intermediates react with peroxide, they produce a strong and sustained chemiluminescent signal that can be captured by x-ray film ccd imaging. The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers. Pierce ecl 2 substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or pvdf membrane when probed with appropriate primary and secondary antibody concentrations. A broad dynamic range and excellent sensitivity combined with a long-lasting signal make pierce ecl 2 substrate a desirable western blotting substrate.Highlights:high sensitivity — detect targets down to the low-picogram level long signal duration — sustained light output for as long as five hours more imaging options — x-ray, ccd or laser-based imagers more affordable — high quality and performance at a lower price than other suppliers' similar substrate