Neutravidin Protein, Maleimide-Activated, Purified Neutravidin Protein For Western Blot, Elisa Or Other Biotin-Based Detection Methods, Each

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Thermo scientific™ neutravidin protein is a specially-prepared form of avidin biotin-binding protein that decreases background in western blotting and elisa; available items include standard hrp, ap and fluorescent conjugates for biotin-based assays.Neutravidin protein is deglycosylated native avidin from egg whites. Removal of the excess carbohydrate by an exclusive process yields a protein with a more neutral isoelectric point and less nonspecific binding properties. Purified and conjugated forms of neutravidin protein provide exceptional performance in western blot, elisa and ihc applications that require biotin-binding probes. Assay specificity, sensitivity, and signal-to-noise ratios with neutravidin protein are generally equivalent or better than with the significantly more expensive streptavidin. Highlights:near-neutral isoelectric point – pi equals 6.3, More neutral than native avidinnearly devoid of glycosylation – decreased possibility of lectin binding compared to native avidinno ryd recognition sequence – no known off-target binding domains like streptavidinaffordable – significantly less expensive than streptavidinavailable in multiple formats – choose purified neutravidin protein, prepared hrp and ap conjugates or activated forms for a variety of technique