Pvuii, Hc (50 U/ΜL), Fermentas Pvuii Is Available As A Fastdigest Enzyme Which Is Used For Rapid Dna Digestion, Each

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Thermo scientific™ pvuii is available as a fastdigest enzyme which is used for rapid dna digestion, more than 90% of the digested dna fragments can be ligated and more than 95% of these sites can be recut at a 5′-termini concentration of 0.15 μM after a 10-fold overdigestion (5u/μg dna x 2 hours) with pvuii.5'...C a g▵c t g...3'3'...G t c▵g a c...5'Conditions for 100% activity1x buffer g:10mm tris-hcl (ph 7.5 At 37°c), 10mm mgcl2, 50mm nacl and 0.1Mg/ml bsaincubate at 37°cstorage bufferpvuii is supplied in:10mm tris-hcl (ph 7.4 At 25°c), 100mm kcl, 1mm dtt, 1mm edta, 0.2Mg/ml bsa and 50% (v/v) glycerolligation and recleavageafter 10-fold overdigestion with pvuii, more than 90% of the dna fragments can be ligated and more than 95% of these can be recutmethylation effectsdam: never overlaps —no effectdcm: never overlaps —no effectcpg: never overlaps —no effectecoki: never overlaps —no effectecobi: may overlap —effect not determineddigestion of agarose-embedded dnaminimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda dna in 16hours note:greater than 15-fold overdigestion with pvuii may result in star activity.