Rack1 Mouse, Unlabeled, Clone: 20, Bd, Mouse Monoclonal Antibody, Each

Details:

Several proteins which specifically bind to pkc have been classified as racks (receptors for activated c-kinase). Rack1 was cloned from a rat brain cdna expression library by screening for proteins that bind pkc in the presence of phosphatidylserine, diacylglycerol, and calcium in a pkc overlay assay. By sequence homology, rack1 appears to belong to a superfamily that includes the β subunit of g proteins. All of these proteins contain five to eight internal repeat elements known as wd40 motifs, which appear to have a role in protein-protein interactions. In addition, rack1 contains two short sequences homologous to a pkc-binding sequence identified in annexin i and in the brain pkc inhibitor kcip. The binding of rack1 to pkc is dose-dependent and occurs at a site on pkc that is distinct from the catalytic domain, indicating that rack1 is not a pkc substrate.Immunofluorescence, immunohistochemistry, immunoprecipitation, western blotting