Shc Mouse, Unlabeled, Clone: 30, Bd, Mouse Monoclonal Antibody, Each

Details:

The mammalian shc proteins, which are expressed as multiple isoforms (46, 52, and 66kda), each contain a c-terminal sh2 domain and an n-terminal glycine/proline rich sequence. These proteins function as early signaling intermediates (also called adaptor proteins) which relay g protein coupled receptor (gpcr) and receptor tyrosine kinase (rtk)-induced signals via the ras transduction pathway. To this end, the shc proteins contain specific tyrosine residues which are phosphorylated following association with the active rtks. Phosphorylated shc forms a complex with the adaptor protein grb2. Association of the shc-grb2 complex with the ras guanine nucleotide exchange factor (ras-gef) mediates the localization of ras-gef to the plasma membrane. Once at the plasma membrane, ras-gef activates ras by catalyzing the ras-gtp for ras-gdp exchange. Over-expression of shc results in cell transformation, and phosphorylation of shc correlates with activation of the erk1/erk2 kinases. The shc proteins are mediators of signals that are essential for cell metabolism, growth, and differentiation.Immunofluorescence, western blotting