Sigma Dl-Dithiothreitol, =99.0% (Rt), 5 G, Each

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Specifications synonyms: threo-1,4-dimercapto-2,3-butanediol; cleland's reagent; dttformula: hsch2ch(oh)ch(oh)ch2shformula weight: 154.25Cas no.: 3483-12-3Purity: ≥99.0% (Rt)storage temperature: 2-8°capplication: an excellent reagent for maintaining sh groups in reduced state; quantitatively reduces disulfides. Dtt is effective in sample buffers for reducing protein disulfide bonds prior to sds-page. Dtt can also be used for reducing the disulfide bridge of the cross-linker n,n′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. Dtt is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of dtt (100 mm) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mm).Anion traces: sulfate (so42-): ≤50 mg/kgassay: ≥99.0% (Rt)cation traces: mn: ≤5 mg/kgimpurities: ≤0.3% 4,5-Dihydroxy-1,2-dithianemp: 42-43°csolubility: h2o: soluble50 mg/ml, clear, colorlessstorage temp.: 2-8°C