Taqman Reverse Transcription Reagents, For Use With Reverse Transcriptase-Pcr (Rt-Pcr), Each

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Random hexamers, oligo (dt)16, and reverse primers are included for cdna synthesis flexibility.Contain multiscribe reverse transcriptase, a recombinant moloney murine leukemia virus reverse transcriptase (mmlv rt)random hexamers and oligo (dt)16 included for flexibilityindividual components provide versatility in assay set-upcompatible with one-step or two-step rt-pcr reactionsoptimal reaction temperature: 42°cfinal product size: 5kb or lessusing taqman reverse transcription reagents taqman reverse transcription reagents provide all the necessary components to perform the reverse transcription of rna to cdna. For two-step rt-pcr, random hexamers, oligo (dt)16, and sequence-specific reverse primers can all be used. The choice of primers for reverse transcription is best made after experimentally evaluating all three priming systems.Built-in normalization the taqman reverse transcription reagents contain a passive internal reference to normalize non-pcr-related fluorescence fluctuations. Normalizing with a passive internal reference minimizes well-to-well variability that can result from a variety of causes, such as pipetting errors and sample evaporation. The passive reference is also essential for accurate results when multiple probes (with different reporter dyes) are combined in a single tube.Applications:pcr & real-time pcr, reverse transcriptio