Tef-1 Mouse, Unlabeled, Clone: 31, Bd, Mouse Monoclonal Antibody, Each

Details:

Tef-1 (transcription enhancer factor-1) is a member of a family of transcription factors that contain an evolutionarily conserved dna binding domain (dbd), tea/atts. Tef-1 was identified as a hela cell transcriptional activator that bound, via its tea domain, to the gt-iic and sph enhancer elements. Comparison of various enhancers indicates that tef-1 binds to highly degenerate dna sequences and cooperatively interacts with tandem repeats of its binding sites, which increases its enhancer activity. However, the dna binding activities of tef-1 are modulated by sequences outside the dbd. The n-terminal basic portion of tef-1 contains the dbd. The c-terminal portion contains the transactivation domain and a zinc finger-like motif that is thought to be involved in protein dimerization or interaction with additional transcription factors. Tef-1 induced transcription requires a limiting transcriptional intermediary factor (tif) and a tata-binding protein associated factor (htafiis). Although tef-1 is expressed ubiquitously during embryogenesis, its expression is limited to adult kidney, heart, brain, skeletal muscle, and lungs. Disruption of murine tef-1 gene results in fetal death.Immunofluorescence, western blotting