X-gal, 250mg, Each

Details:

Chromogenic substrate of β-galactosidase used in combination with iptg for detection of β-galactosidase activity in bacterial colonies. X-gal is cleaved at the b1-4 bond between galactose and the 5-bromo-4-chloro-3-indolyl portion of x-gal by β-galactosidase via hydrolysis. The cleavage of x-gal results in the production of a water insoluble blue dichloro-dibromo-indigo precipitate. In cloning strategies with vectors such as lambda-11, m13mp18 and 19, puc18 and 19, pur222 the e. Coli lacz gene is transformed to lac cells. After transformation the cells show β-galactosidase activity in the presence of media containing x-gal and iptg. The insertion of a dna fragment into the cloning sites of the lacz gene results in the disruption of β-galactosidase activity leading to the appearance of white colonies. Non-recombinant cells produce a blue indigo dye.Water content: 0.05% (K. Fisher) protect from light and moisture.