X-Gluc, Effectively Screen Colonies With X-Gluc, A Substrate For ß-Glucuronidase (Gus) Which Is Encoded By Gusa, A Widely Used Reporter Gene, Each

Details:

X-gluc (5-bromo-4-chloro-3-indolyl-beta-d-glucuronic acid, cyclohexylammonium salt) is a substrate for beta-glucuronidase (gus) which is encoded by gusa, a widely used reporter gene. Glucuronidase cleaves x-gluc to produce colorless glucuronic acid and an intense blue precipitate of chloro-bromoindigo (see the scheme below).Glucuronidase cleaves x-gluc to produce colorless glucuronic acid and an intense blue precipitate of chloro-bromoindigo (see the scheme below). Recommended for:detection of gus expression in plant cells and tissues (1)detection of infections caused by e.Colidetection of bacterial contamination in food and water samples note:preparation of a 0.1M stock solution in dimethylformamide or dimethylsulfoxide is recommended.