Zap-70 Kinase Mouse, Unlabeled, Clone: 29, Bd, Mouse Monoclonal Antibody, Each

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Zap-70 is a protein tyrosine kinase (ptk) that associates with the ζ subunit of the t cell antigen receptor (tcr) and undergoes tyrosine phosphorylation following tcr stimulation. Zap-70 contains two sh2-like domains with the ptk domain being at the c-terminus. It appears that both zap-70 and syk are recruited to the phosphorylated cd3 and ζ subunits after tcr stimulation. The src-family ptks, lck, and fyn, seem to play a role upstream of zap-70 and syk where they phosphorylate cd3 and ζ. Thus both src- and zap-70/syk-family ptks are critical for efficient induction of tyrosine phosphoproteins in heterologous cells. The significance of zap-70 in mediating tcr signal transduction has been confirmed by showing that zap-70 activity is absent in an autosomal recessive form of severe combined immunodeficiency (scid). This is due to mutations affecting the zap-70 kinase domain which affect the stability of the protein. The levels of lck and fyn appear to be normal in the same cells. Results of the zap-70 mutations in these patients include an unusual absence of peripheral cd8+ t cells and an abundance of cd4+ t cells in peripheral lymphoid organs. These results indicate that these two populations of t cells develop through utilization of different intracellular signaling pathways.Immunofluorescence, immunohistochemistry, immunoprecipitation, western blotting